Cytotoxic and genotoxic potential of surface water from the Pitimbu ... In this study, the onion (Allium cepa) root test was used to evaluate the ... 1988), and have been used for monitoring the potential synergistic effects of mixtures ... sedimentation, filtration, chlorination with sodium hypochlorite …

concentration of 0.2, 0.4 and 0.6 mg/ml (three controls). Cytogenetic analysis included monitoring the effects of different concentrations of tested UV filter in different concentrations of free chlorine, to the mitotic activity of meristem cells of Allium cepa L., and the types of genotoxic effects expressed during the cell cycle as well. The

Onion ( Allium cepa L.) belonging to the family of Amaryllidaceae, is the second most important horticultural crop in the world after tomatoes, which is cultivated throughout the world and is extensively grown and widely consumed in India [10]. Onion seeds are convex one side and flattened on other side and are covered by a black seed coat.

A genotoxic effect was found for sodium hypochlorite in all plant tests, at both TOCs considered, ... All disinfectants determined genotoxic effects in Allium cepa tests. ... did not induce a greater genotoxic effect than 2.5 mg/L TOC concentration samples. Also, low TOC concentration, similar to ground water, may react with disinfectants to ...

The pars are means ± SE. concentration up to 250 pmm to 1000 ppm was deacresed the fresh weight and from analytical side, Dunnett test indicated high significant between control and 500 and 1000 ppm of butenolide. Figure3: The effect of butenolide on merstimic cells of Allium cepa L. root tips.

In the T. harzianum-free According to results, it is clear that there is a possitive treatment, increased salinity levels resulted in decreased effect of T. harzianum on during germination stages of germination, inhibition of seedling growth and increased onion (Allium cepa L.) seeds under salt stress conditions.

In vitro Antifungal Activity of Some Plant Essential Oils. The maximum inhibition of the mycelial growth was recorded in E. globulus which completely inhibited the mycelial growth at 250 and 500 ppm concentrations and LD 50 of this oil was 42.45 ppm L-1.Also, A. cepa essential oil was completely inhibited the mycelial growth of F. solani but at 500 ppm concentration.

Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro.

Initiation of embryogenic suspension culture and plant regeneration in onion (Allium cepa L.) S Tiwari 1*, M K Tripathi 2, ... for 1 min followed by a treatment of 5% (v/v) sodium hypochlorite for 15 min and finally 4-5 times rinsing with sterilized deionized water under asepsis. Seeds

The study was undertaken to develop an efficient protocol for in vitro regeneration of shallot ( Allium cepa ). Two local shallot varieties (Huruta and Minjar) were used as experimental materials where basal discs were used as explants. Murashige and Skoog medium supplemented with different concentrations and combinations of 2, 4-Dichlorophenoxyacetic Acid, 6-Benzylaminopurine, Kinetin and α ...

Allium cepa x Allium fistulosum, leaf base, leaf extension, hot water dips, sodium hypochlorite Abstract. Extension growth of minimally processed (removal of roots and compressed stem) green onions (Allium cepa L. x A. fistulosum L.) was greatly reduced by storage in ... determine the effect …

Background and Objective: There have been a number of reported drawbacks and efficacy issues regarding the use of bleaching agents in the plant industry. This study was conducted to determine the cytological effects of the bleaching agent (Quneex) on the plant cells and plant DNA using the Allium cepa assay.Materials and Methods: It was subjected sixteen root meristems of A. cepa to different ...

Alleviation of Adverse Effects of Copper on Allium cepa L. …. 3 subsequently electrical conductivity of the solution (EC1) was determined.Samples were then autoclaved at 120 C for 20 min and the final electrical conductivity (EC2) was obtained after equilibration at 25 C. Leaf cell MSI was determined according to the following formula

through Allium cepa assay. Five concentrations of 1250, 1000, 750, ... sodium hypochlorite then rinsed with distilled water thrice Cleaned samples ... indicative of the strong genotoxic effect. The higher the concentration of the extract is, the higher the aberrations were in the cells. However, higher aberrations percentage were observed in 50%

Pruebas de ecotoxicidad para establecer el potencial genotóxico del hipoclorito de sodio, mediante bulbos de cebolla Allium cepa L y semillas de lechuga Lactuca Sativa L como bioindicadores

Keywords: Sodium hypochlorite, Physiological parameters, Chromosomal aberration Abstract The effects of sodium hypochlorite (NaClO) on the seed germination, seedling growth (radicle length, radicle number and fresh weight), mitotic activity and chromosomal aberrations of Allium cepa L. germinated under salt stress were studied.

Jan 24, 2018· The aim of this study was to determine the antimicrobial capacity, minimum inhibitory concentration (MIC), and cytotoxic effects of a Peganum harmala seed extract in comparison to 5.25% sodium hypochlorite (NaOCl). The oral pathogen Enterococcus faecalis was used to evaluate the antimicrobial capacity, and the MIC values were determined through serial dilution.

In Vitro Plantlet Regeneration from the Bulbs of Shallot (Allium Cepa Var. Group Aggregatum) ... Allium cepa var. ascalonicum [16,17,18,19] and Allium scorodoprasum spp rotundum [20]. An established ... surface sterilized in 5.25% sodium hypochlorite ((NaOCl) solution for 15 min and again rinsed four times in

and RUTTLE (1938), who first studied the effect of colchicine on plant cells (stamen hairs of Tradescantia), arrived at a similar result. The material of the experiments dealt with in the present paper consists of root tips of Allium fistulosum and Cepa. Root tips of A. fistulosum were secured from bulbils that had overwintered in the

The results in S.cerevisiae show a genotoxic response on the end-points considered with an effect only at doses higher (5- to 10-fold) than the concentration normally used for water disinfection; sodium hypochlorite and peracetic acid are able to induce genotoxic effects without endogenous metabolic activation (in stationary phase cells ...

October 2012 to study the influence of mycorrhizal fungi on antioxidant activity in onion (Allium cepa L.) at the research station of Tabriz University, Tabriz, Iran. After disinfection of onion seeds with sodium hypochlorite (1%) for 10 minutes they were sown in a sandy loam …

The effect of collected TE on Allium cepa showed a significant reduction in root length, decrease in dry weight, increase in root diameter, and increase in root hairs. The estimated EC 50 value of Allium cepa exposed to TE was found to be 7.79% which is almost similar to the findings of Gupta et al. (2011) [40].

Plant material. Seeds of Allium cepa L. (from horticulture farm in Lubiczów) were sterilized using sodium hypochlorite (0.3 % v/v), sown on moist filter paper and germinated at room temperature. Selected dark-grown seedlings with primary roots reaching 1.5 ± 0.2 cm were cultivated at 20 °C in Petri dishes (Ø 6 cm) filled with 10 mL of either distilled water (control samples) or 0.75 mM HU ...

The results showed that Allium cepa L. is a species that offers an experimental model to evaluate the cytotoxic effect, in this case sodium hypochlorite, which generated cellular abnormalities ...

Biomass, Flavonol Levels and Sensory Characteristics of Allium cultivars Grown Hydroponically at Ambient and Elevated CO 2 Leslie Thompson Animal and Food Sciences, Texas Tech University, Lubbock TX ... 50-ppm sodium hypochlorite solution and drained, they

Study the effect of the type of packaging and treatment sodium hypochloride and licorice extract in percentages of weight loss and total damage in class onion taxas early grano. ... Allium cepa allium cepa Subject Category: Organism Names see more details, ... sodium hypochlorite. storage quality. temperature. Show more.

May 30, 2009· Chlorination is widely used method in the disinfection of drinking and utility water worldwide. In this study, cytotoxic and genotoxic effects of sodium hypochlorite were investigated by the cytokinesis-block micronucleus assay and chromosomal aberration analysis on human peripheral lymphocytes in vitro.

May 30, 2019· The objective of this study was to evaluate the cytotoxic effect of different sodium hypochlorite concentrations, using apical root cells of P. sativum as a bioindicator. Initially, the seeds of P. sativum were exposed to different concentrations of sodium hypochlorite (0.1, 0.2, 0.4, 0.8, 1.6, 2 mg L −1) and to a control solution based on distilled water.